《植物生理学报》 2009, 45(12): 1167-1170

AgNO₃对网纹甜瓜试管苗再生和玻璃苗产生的影响

齐红岩*, 陈岩, 刘雪超

沈阳农业大学园艺学院, 辽宁省设施园艺重点实验室, 沈阳110866

通信作者：齐红岩；E-mail: hyqiaaa@126.com；Tel: 024-88487166

摘　要：

以网纹甜瓜品种‘ 中蜜1号’ 为试材, 在其不同培养阶段添加不同浓度AgNO3, 检测不定芽的诱导率和增殖率、玻璃苗发生率和生根情况的结果表明, 添加AgNO3后, 子叶的愈伤组织诱导率下降, 添加70 μmol·L-1 AgNO3可促进不定芽的诱导, 50~60 μmol·L-1 AgNO3 明显促进不定芽增殖, 并完全抑制玻璃苗的产生, 添加50 μmol·L-1 AgNO3 的生根最好。

关键词：网纹甜瓜; 试管苗再生; 玻璃化; AgNO3

收稿：2009-08-03   修定：2009-10-12

资助：国家科技支撑计划(2006BAD07B04)。

Effect of AgNO₃ on Plantlet Regeneration and Vitrification of Netted Melon (Cucumis melo L.) in vitro

QI Hong-Yan*, CHEN Yan, LIU Xue-Chao

Key Laboratory of Protected Horticulture of Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: QI Hong-Yan; E-mail: hyqiaaa@126.com; Tel: 024-88487166

Abstract:

The netted melon ‘No.1 Zhongmi’ was used in this experiment. Adventitious shoot induction, proliferation, vitrification and rooting of plantlets were investigated in different culture stages with different AgNO₃ concentrations. The results showed that cotyledon callus induction rate was inhibited with AgNO₃addition. Adventitious shoot induction was promoted by addition of 70 μmol·L^-1 AgNO₃. Adventitious shoot proliferation was improved obviously by 50–60 μmol·L^-1 AgNO₃ and vitrification shoot formation was also inhibited completely. Root induction was significantly improved by 50 μmol·L^-1 AgNO₃.

Key words: netted melon (Cucumis melo L.); plantlet regeneration; vitrification; silver nitrate